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The Molecular Systematic Of Shads (Alosa spp.) in Turkish Seas

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      <entry>http://traglor.cu.edu.tr/objects/objectFile/X5YgbL5Z-4102013-24.pdf</entry>
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    <title>
      <string language="tr">Türkiye Denizlerindeki Tirsilerin (Alosa spp.) Moleküler Sistematiği</string>
      <string language="en">The Molecular Systematic Of Shads (Alosa spp.) in Turkish Seas</string>
      <string language="de">Molekularen systematischen von Gummifischen (Alosa spp.) in türkischen Gewässern</string>
    </title>
    <language>tr</language>
    <description>
      <string language="tr">Bu çalışmada Türkiye denizlerinde bulunan 5 tirsi türü (Alosa caspia, Alosa fallax nilotica, Alosa maeotica, Alosa pontica, Alosa tanaica) arasındaki genetik ilişkinin şekli ve derecesi mtDNA PCR-RFLP yöntemi kullanılarak analiz edilmiştir. MtDNA'nın ND 5/6 gen bölgesi PCR yöntemi ile çoğaltılarak 6 sınırlama enzimi BsurI, AluI, EheI, Hin6I, RsaI, XhoI) ile kesilmiştir. RFLP sonucunda 5 tirsi türünde toplam 13 haplotip saptanmıştır. Türlerin içerisinde ortalama haplotip çeşitliliği 0.6000 ve nükleotit çeşitliliği 0.005331 olarak bulunmuştur. Türlerin kendi aralarında karşılaştırılmalarında ise ortalama nükleotit çeşitliliği 0.008024 ve ortalama nükleotit farklılığı 0.002693 olarak gözlemlenmiştir. Türler arasındaki genetik uzaklık değerlerine bakıldığında en yüksek değerin A. pontica ile A. caspia arasında 0.009599'luk bir değer olduğu, en düşük değerin ise, A. maeotica ve A. tanaica türleri 0.000007 arasında olduğu görülmektedir. Monte-Carlo (X2) İkili Karşılaştırma Analizi sonucu bütün türler arasında önemli derecede farklılıklar olduğu belirlenmiştir (p&lt;0.001). UPGMA soyağacı analizine göre, A. tanaica ve A. maeotica türleri birbirine genetik olarak en yakın bulunmuş, A. pontica ise türler içerisinde en farklı tür olarak göze çarpmıştır</string>
      <string language="en">In this study mitochondrial DNA polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was used to analyse the phylogenetic relationships among five shad species (Alosa caspia, A. fallax nilotica, Alosa maeotica, Alosa pontica, Alosa tanaica) from Turkish seas. The complete NADH 5/6 gene region of mtDNA amplified by PCR were digested with six restriction enzymes, BsurI, AluI, EheI, Hin6I, RsaI, XhoI respectively. A total of 13 haplotypes were detected from five shad species. The average haplotype diversity and nucleotid diversity within species were 0.6000 and 0.005331 respectively. The average nucleotid diversity and nucleotide divergence among species were 0.008024 and 0.002693 respectively. The highest genetic divergence was observed between A. pontica to A. caspia (0.009599) and lowest, between A. maeotica and A. tanaica (0.000007). Monte Carlo (X2) pairwise found determined significant differences between all species, depending on the mtDNA regions examined (p&lt;0.001). In the UPGMA (Unweighhed Pair Group Method) dendogram, A. tanaica and A. maeotica exhibited closest genetical similarity and A.pontica showed highest genetical differantiation within Alosa genus</string>
      <string language="de">In dieser Studie mitochondriale DNA-Polymerase Kettenreaktion-Beschränkung Fragment Länge Polymorphie war (PCR-RFLP) Assay verwendet, um die phylogenetischen Beziehungen zwischen fünf Shad-Arten (Alosa Caspia, A. Fallax Nilotica Maeotica Alosa Alosa Pontica, Alosa Tanaica) von türkischen Meeren zu analysieren. Die komplette NADH 5/6-gen Region MtDNA durch PCR verstärkt wurden mit sechs Restriktionsenzyme, BsurI, AluI, EheI, Hin6I, RsaI, XhoI bzw. verdaut. Insgesamt 13 Haplotypen wurden von fünf Shad Arten festgestellt. Die durchschnittliche Haplotype Vielfalt und Nucleotid Vielfalt innerhalb der Arten betrugen 0,6000 und 0.005331. Die durchschnittliche Nucleotid-Vielfalt und Nukleotid-Divergenz bei den einzelnen Spezies waren 0.008024 und 0.002693. Die höchste genetische Divergenz wurde zwischen A. Pontica A. Caspia (0.009599) und niedrigste, zwischen A. Maeotica und A. Tanaica (0.000007) beobachtet. Monte-Carlo (X 2) paarweise gefunden bestimmt signifikante Unterschiede zwischen allen Arten, abhängig von der MtDNA-Regionen untersucht (p &lt; 0,001). Im UPGMA (Unweighhed-paar-Gruppe-Methode) Dendogram, A. Tanaica und A. Maeotica ausgestellt am nächsten genetische Ähnlichkeit und A.pontica zeigte höchste genetische Differantiation Alosa Gattung</string>
    </description>
    <keyword>
      <string language="tr">Türkiye denizleri</string>
      <string language="en">Turkish seas</string>
      <string language="de">Türkischen Meeren</string>
    </keyword>
    <keyword>
      <string language="tr">tirsi türleri</string>
      <string language="en">shad species</string>
      <string language="de">Shad-Arten</string>
    </keyword>
    <keyword>
      <string language="tr">mitokondrial dna</string>
      <string language="en">mitochondrial dna</string>
      <string language="de">Mitochondriale Dna</string>
    </keyword>
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      <string language="tr">pcr-rflp</string>
      <string language="en">pcr-rflp</string>
      <string language="de">Pcr-Rflp</string>
    </keyword>
    <keyword>
      <string language="tr">moleküler sistematik</string>
      <string language="en">molecular systematic</string>
      <string language="de">Molekulare Systematische</string>
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FN: Deniz Ergüden      
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